Identification of aryl hydrocarbon receptor 2 in aquatic birds; cDNA cloning of AHR1 and AHR2 and characteristics of their amino acid sequences     

Yasui T  Kim EY  Iwata H  Tanabe S 《Marine environmental research》2004,58(2-5):113-118

The toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and its related planar halogenated aromatic hydrocarbons (PHAHs) are mediated by the aryl hydrocarbon receptor (AHR). To investigate the potential sensitivity to PHAHs and the evolutional diversity of AHR in aquatic birds, AHR cDNAs were initially cloned and sequenced from the livers of a black-footed albatross (Diomedea nigripes) and a common cormorant (Phalacrocorax carbo). In this study, we report the identification of two distinct AHR paralog genes in these species. The two full-length AHR cDNAs from albatross were highly divergent (33% overall amino acid identity, and 60% identity in the N-terminal half). Phylogenetic analysis showed that one of them belongs to the AHR1 clade and the other one to the AHR2 clade, which has been identified only from fishes, but not yet from mammals and birds. Albatross AHR1 encoded a 861-residue protein with a predicted molecular mass of 96.7 kDa, and in the case of albatross AHR2, 925 amino acids and 100.7 kDa. From cormorant liver, the full-length AHR1 cDNA and the partial AHR2 cDNA were cloned. This result strongly suggests that bird species also possess two distinct AHR genes (AHR1 and AHR2). To our knowledge, this is the first report on the presence of an AHR2-like isoform in bird species as well as in fish.  相似文献   

Fluctuation of dissolved organic carbon in seawater of Sagami Bay during 1971–1972     

Norio Ogura  Akiyoshi Kamatani  Nobutada Nakamoto  Masaki Funakoshi  Shizuo Iwata 《Journal of Oceanography》1975,31(1):43-47

Fluctuation of dissolved organic carbon (DOC) was studied during 1971–1972 at monthly intervals in surface layers of Sagami Bay. Concentration of DOC varied from 0.8 to 1.7 mgC/l in surface water (0 m). Maximum concentration of 1.7 mgC/l was observed in July 1971 and after then DOC decreased gradually to a minimum of 0.8 mgC/l in May 1972. The fluctuation of DOC during the observation periods seems to have close relations with those of water temperature and salinity. High DOC concentration found in summer months may be associated with bloom of phytoplankton or intrusion of seawater from Tokyo Bay and/or inland water containing high DOC.  相似文献   

Influence of Source/Sink Distributions on Flux–Gradient Relationships in the Roughness Sublayer Over an Open Forest Canopy Under Unstable Conditions     

Hiroki Iwata  Yoshinobu Harazono  Masahito Ueyama 《Boundary-Layer Meteorology》2010,136(3):391-405

The flux–gradient relationships in the unstable roughness sublayer (RSL) over an open canopy of black spruce forest were examined using long-term observations from an instrumented tower. The observed gradients normalised with the surface fluxes and height above the zero-plane displacement showed differences from a universal function established in the surface layer. The magnitude of differences was not constant throughout the year even at the same observation height. Also the magnitude of the differences was different for each scalar, and scalar similarity in the context of the flux–gradient relationship did not always hold. The variation of the differences was explained by the relative contribution of overstorey vegetation to the total flux from the entire ecosystem. This suggests that a mismatch of the vertical source/sink distributions between scalars leads to a different strength of the near-field dispersion effect for each scalar, and this resulted in inequality of eddy diffusivity among scalars in the RSL. An empirical method that predicts the magnitude of differences is proposed. With this method, it is possible to estimate the eddy diffusivity of scalars provided that the relative contribution of overstorey vegetation to the total flux from the ecosystem is known. Also this method can be used to estimate the eddy diffusivity for scalars whose primary sources are at ground level, such as methane and nitrous oxide.  相似文献   

A seasonal effect in diurnal variation of the atmospheric electric field on the Pacific coast of Japan     

M. Takagi  A. Iwata 《Pure and Applied Geophysics》1980,118(2):953-963

The measurement of the atmospheric electric field was carried out with a field mill at a small island in a bay on the Pacific coast of Japan. The data obtained during 80 months from 1971 to 1977 were analysed. It was found that characteristically the diurnal variation regularly alters with the seasons. Whereas the electric field in winter has the same diurnal pattern as that observed at globally representative stations, it exhibits in summer a pattern depending on the variation of the local electric conductivity. The alteration is inferred to be associated with regional atmospheric conditions surrounding the observation site.  相似文献   

S-wave velocity structures of sediments estimated from array microtremor records and site responses in the near-fault region of the 1999 Chi-Chi,Taiwan earthquake     

Satoh?ToshimiEmail author  Hiroshi?Kawase  Tomotaka?Iwata  Sadanori?Higashi  Sato?Toshiaki  Huey-Chu?Huang 《Journal of Seismology》2004,8(4):545-558

The 1999 Chi-Chi, Taiwan earthquake, M_W = 7.6, caused severe damage in the near-fault region of the earthquake. In order to evaluate site effects in the near-field strong motions we estimate S-wave velocity structures of sediments at four sites using array records of microtremors. We also recalculated S-wave velocity structures at other four sites previously reported. To show the validity of the estimated S-wave velocity structures we separate empirical site responses from aftershock records using the generalized inversion method and show the agreement between empirical and theoretical site responses. We also show an observed fact that suggests soil nonlinearity during the Chi-Chi earthquake by comparing horizontal-to-vertical spectral ratios (HVRs) for main shock records with HVRs for aftershock records. Then we calculate one-dimensional equivalent-linear site responses using the estimated S-wave velocity structures and the main shock records observed on the surface. It is found that site amplification due to thick (about 6 km) sediments is one of the important factors for explaining the long-period velocity pulses of about 5 to 10 sec observed at sites in the footwall during the Chi-Chi earthquake. It is also found that the theoretical site responses of shallow soft sediments at sites that sustained severe damage in the hanging wall shows significant amplification around 1 sec. As the amplitude of velocity pulses with period around 1 sec is most critical in causing damage to ordinary buildings of moderate heights, our results suggest that the 1-sec period velocity pulses, amplified by the site response of shallow sediments should contribute to the severe damage during the Chi-Chi earthquake.  相似文献   

Accumulation of persistent organochlorines in resident white-breasted waterhens (Amaurornis Phoenicurus) from Cambodia     

Monirith I  Kunisue T  Iwata H  Tanabe S  Seang Tana T 《Marine pollution bulletin》2003,46(10):1341-1348

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Molecular cloning and mRNA expression of cytochrome P4501A1 and 1A2 in the liver of common minke whales (Balaenoptera acutorostrata)     

Satoko Niimi  Michio X. Watanabe  Eun-Young Kim  Hisato Iwata  Genta Yasunaga  Yoshihiro Fujise  Shinsuke Tanabe 《Marine pollution bulletin》2005,51(8-12):784

This study presents full-length cDNA sequences of CYP1A1 and 1A2, in common minke whale (Balaenoptera acutorostrata) from the North Pacific. Both CYP1A1 and CYP1A2 cDNAs had an open reading frame of 516 amino acid residues, and predicted molecular masses were 58.3 kDa and 58.1 kDa, respectively. The deduced full-length amino acid sequence of CYP1A1 revealed higher identities with those of sheep (86%) and pig (87%), and that of CYP1A2 was most closely related to human (82%) and monkey CYP1A2 (82%) among species from which CYP1A2 has been isolated so far. Differences in certain conserved and functional amino acid residues of CYP1A1 and 1A2 between common minke whale and other mammalian species indicate the possibility of their specific metabolic function. Concentrations of organochlorine compounds (OCs) including PCBs and DDTs analyzed in common minke whale liver showed no significant correlation with hepatic mRNA expression levels of CYP1A1 and CYP1A2, indicating no induction of these enzymes by such OCs.  相似文献   

Aircraft measurements of oxides of nitrogen along the eastern rim of the Asian continent: Winter observations     

Y. Kondo  W. A. Matthews  A. Iwata  Y. Morita  M. Takagi 《Journal of Atmospheric Chemistry》1987,5(1):37-58

Aircraft observations of oxides of nitrogen (NO _y ), measured with a ferrous sulfate converter, over the sea surrounding the Japanese islands (30–43° N, 131–141° E) were carried out in the winter of 1983 and 1984 at altitudes mostly between 3 and 8 km. NO _y defined here is the sum of NO, NO₂, and other unstable oxides of nitrogen that are converted to NO by ferrous sulfate. The main observations were:

1. Over the Pacific Ocean between the latitudes of 30–35° N, the observed NO _y mixing ratio between 3 and 8 km was a fairly constant 200 pptv. The NO mixing ratio increased with altitude from 15 pptv at 3 km to 35 pptv at 7 km.
2. Over the Sea of Japan, tropospheric NO _y mesured between 1 and 6 km started increasing with latitude North of 35° N and reached about 1000 pptv at 40° N.
3. NO _y was measured in an air mass transported from the stratosphere near a tropopause fold region. When the ozone mixing ratio was between 80 and 140 ppbv, the NO _y mixing ratio was about 200 pptv.

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Organotin residues and the role of anthropogenic tin sources in the coastal marine environment of Indonesia     

Sudaryanto A  Takahashi S  Iwata H  Tanabe S  Muchtar M  Razak H 《Marine pollution bulletin》2005,50(2):226-235

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Concentrations of trace elements in marine fish and its risk assessment in Malaysia   总被引：1，自引：0，他引：1  

Agusa T  Kunito T  Yasunaga G  Iwata H  Subramanian A  Ismail A  Tanabe S 《Marine pollution bulletin》2005,51(8-12):896-911

Concentrations of trace elements (V, Cr, Mn, Co, Cu, Zn, Ga, Se, Rb, Sr, Mo, Ag, Cd, Sn, Sb, Cs, Ba, Hg, Tl, Pb and Bi) were determined in muscle and liver of 12 species of marine fish collected from coastal areas in Malaysia. Levels of V, Cr, Mn, Co, Cu, Zn, Ga, Sr, Mo, Ag, Cd, Sn, Ba and Pb in liver were higher than those in muscle, whereas Rb and Cs concentrations showed the opposite trend. Positive correlations between concentrations in liver and muscle were observed for all the trace elements except Cu and Sn. Copper, Zn, Se, Ag, Cd, Cs and Hg concentrations in bigeye scads from the east coast of the Peninsular Malaysia were higher than those from the west, whereas V showed the opposite trend. The high concentration of V in the west coast might indicate oil contamination in the Strait of Malacca. To evaluate the health risk to Malaysian population through consumption of fish, intake rates of trace elements were estimated on the basis of the concentrations of trace elements in muscle of fish and daily fish consumption. Some specimens of the marine fish had Hg levels higher than the guideline value by US Environmental Protection Agency (EPA), indicating that consumption of these fish at the present rate may be hazardous to Malaysian people. To our knowledge, this is the first study on multielemental accumulation in marine fish from the Malaysian coast.  相似文献